RESUMO
This study aimed to translate the Patient-Reported Outcomes Measurement Information System (PROMIS) General Life Satisfaction Short Form (GLS SF5a) into the Arabic language and psychometrically validate the scale in the general population of Saudi Arabia. The translation processes followed the international recommendations of the FACIT Measurement System. The study was a multicentre cross-sectional study conducted in Riyadh, Saudi Arabia. A total of 657 individuals who were above 18 years of age and able to write and comprehend Arabic completed the GLS SF5a. Rasch analysis was used to evaluate item fit, reliability indices, item difficulty, principal component analysis and local item dependency. WINSTEPS (v. 5.6.0) was used for the analysis. The translation process and cognitive defibring were completed with no issues. The rating scale categories had a disordered threshold. All items, except one, demonstrated a satisfactory fit to the Rasch model. The reliability of the person separation was 0.86. The scale was unidimensional, and no items showed local dependency. Overall, this study confirms the psychometric properties of the Arabic version of the PROMIS GLS SF5a, which can be used as an instrument for measuring general life satisfaction in the general population. Further research is required to explore responsiveness, interpretability and feasibility in the clinical setting.
RESUMO
A range of natural products have been extensively studied for their chemopreventive potential for cancer, including those that inhibit growth and induce apoptosis. Sidr honey derived from the Ziziphus or Lote tree (Ziziphus spina-christi, Ziziphus lotus, or Ziziphus jujuba) is used in a wide range of traditional medicine practices. In the current study, the Saudi Sidr honey was analyzed by means of a GC-MS chromatogram and investigated for its antiproliferative effects on colorectal cancer cells (HCT-116), breast cancer cells (MCF-7), and lung cancer cells (A-549), as well as its apoptosis induction and cell cycle arrest potentials against human colorectal cancer cells (HCT-116). The effects of Saudi Sidr honey on cells were determined using the MTT assay and the clonogenic assay. The induction of apoptosis was studied using Annexin V-FITC flow cytometry analysis. The propidium iodide staining method was used to detect cell cycle arrest via flow cytometry. By means of performing GS-MS and HR-LCMS analysis, 23 different chemical components were identified from Saudi Sidr honey. A dose-response analysis showed that Saudi Sidr honey was more effective against HCT-116 (IC50 = 61.89 ± 1.89 µg/mL) than against MCF-7 (IC50 = 78.79 ± 1.37 µg/mL) and A-549 (IC50 = 94.99 ± 1.44 µg/mL). The antiproliferation activity of Saudi Sidr honey has been found to be linked to the aggregation of cells during the G1 phase, an increase in early and late apoptosis, and necrotic cell death in HCT-116 cells. Considering these promising findings that highlight the potential use of Saudi Sidr honey as an antitumor agent, further research should be carried out with the aim of isolating, characterizing, and evaluating the bioactive compounds involved in Sidr honey's antiproliferative activity to better understand the mechanism of their action.
